KMID : 1123920060200020442
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Korean Journal of Oriental Physiology and Pathology 2006 Volume.20 No. 2 p.442 ~ p.448
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Single Dose Toxicity Test of ¡¯Mahwangyounpae-tang¡¯ Extract in Male ICR Mouse
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Jung Woo-Sik
Cho Dong-Hee Seo Young-Ho Park Mee-Yeon Choi Hae-Yun Kim Jong-Dea Jeon Kwi-Ok
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Abstract
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To obtain the 50% lethal dose (LD50), approximated lethal dose (ALD) and approximated target organs of ¡¯Mahwangyounpae-tang¡¯ for further study like repeat dose toxicity, genotoxicity and reproductive toxicity, single dose toxicity was tested in male ICR mouse according to KFDA Guideline 1999-61 [KFDA, 1999] at a dosage level of 2,000, 1,000, 500, 250 and . In this study, mortalities, clinical signs, body weight changes and body weight gains, gross findings and weight of principal organs were detected during and/or after 14 days of single dosing. After 2 or 3 days of dosing, 1 or 2 animals in 2,000 and 1,000 mg/kg-dosing groups were died. Excitation and leaping response were observed as test article-treatment related clinical signs. These abnormal signs were restricted to 2,000 and 1,000 mg/kg-dosing groups and they were recovered to normal within 4 days after dosing in case of survivors. A significant decrease of body weight were observed in some periods of observation in 2,000 and 1,000 mg/kg-dosing group from 1 days after dosing compared to those of vehicle control group. A significant decrease of body weight gains were observed in observation periods in 2,000 and 1,000 mg/kg-dosing group compared to those of vehicle control group. Hypertrophy of heart and decoloration of kidney were observed as test article-treatment related gross findings. These abnormal findings were restricted to 2,000 and 1,000 mg/kg-dosing groups. A significant increase of absolute and relative heart and kidney weight were demonstrated in 2,000 mg/kg-dosing groups. LD50 in this study was detected as 2,242.42 mg/kg. ALD in this study was detected as 1,000 mg/kg and the target organ was considered as the heart and kidney.
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KEYWORD
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Single Dose Toxicity, Mahwangyounpae-tang(Ø«üÜëÈøË÷·), ICR Mouse
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